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YFP激发光波长和YFP的发射波长是多少?

作者:时间:2019-09-05 10:33浏览8892 次

信息摘要:

黄色荧光蛋白(Yellow Fluorescent Protein ,YFP)可以看做绿色荧光蛋白的一种突变体,最初来源于维多利亚多管水母( Aequorea victoria)。相对于绿色荧光蛋白,其荧光向红色光谱偏移,而这主要是由于蛋白203位苏氨酸变为酪氨酸。其更大激发波长为514 nm,更大发射波长为527 nm。

YFP激发波长为510nm,更大发射波长为527 nm

黄色荧光蛋白(Yellow Fluorescent Protein ,YFP)可以看做GFP.html' target='_blank' title='绿色荧光蛋白' >绿色荧光蛋白的一种突变体,最初来源于维多利亚多管水母( Aequorea victoria)。相对于绿色荧光蛋白,其荧光向红色光谱偏移,而这主要是由于蛋白203位苏氨酸变为酪氨酸。其更大激发波长为514 nm,更大发射波长为527 nm。

黄色荧光蛋白

 

黄色荧光蛋白的激发波长

黄色荧光蛋白的发射波长

美国路阳生产有多款便携式激发光源,无需荧光显微镜就能轻松观察筛选黄色荧光蛋白表达阳性。便携式YFP激发光源能直接分选YFP表达阳性的植株,能够分选愈伤组织、植株、种子、菌落等。如欲进一步了解便携式荧光蛋白激发光源,可直接联系上海路阳生物技术有限公司的销售客服。

1573530098175899.jpg

上图为黄色荧光蛋白激发光源

1573530166500270.jpg

上图为LUV-40A黄色荧光蛋白观察眼镜

1634021168215779.jpg

上图为LUV-550A 黄色荧光蛋白拍照滤镜

Yellow Fluorescent Proteins/常用黄色荧光蛋白的激发波长和发射波长

YFP种类激发光波长(nm)发射光波长(nm)
EYFP514527
Topaz514527
Venus515528
mCitrine516529
YPet517530
TagYFP508524
PhiYFP525537
ZsYellow1529539
mBanana540553

 

Yellow Fluorescent Proteins

Yellow fluorescent protein (YFP) is a mutant variant of GFP. In this case, a mutation was introduced after the discovery that threonine residue was present near the chromophore in GFP.

To bring stability to the excited dipole moment of chromophore, this threonine residue was mutated. This led to shift by 20 nm in the excitation and emission wavelength of the fluorescent protein, leading to longer wavelengths.

YFP was further improved that led to development of enhanced yellow fluorescent protein or eYFP. This is one of the brightest fluorescent proteins and is widely used for different imaging purposes. GFP and YFP are used in several cases to perform dual imaging, which is a procedure where two molecules are tagged to two different fluorescent proteins. Consequently, they can be imaged together in order to visualize and appraise their inter-dynamics.

 黄色荧光蛋白的激发波长和发射波长

FluorochromeExcitation Wavelength (Nanometers)Emission Wavelength (Nanometers)
Acridine Orange (Bound to DNA)500526
Alexa Fluor 488495519
Alexa Fluor 500503525
Aminofluorescein497519
Astrazon Orange R470540
Aurophosphine450-490515
BCECF (Biscarboxyethylcarboxyfluorescein)505530
BODIPY 492/515 (Difluoroboradiazaindacene)490515
BODIPY 493/503500506
BODIPY 500/510509515
BODIPY 505/515502510
BODIPY FL503512
Calcein494517
Calcium Green506533
CellTracker Green CMFDA (Chloromethylfluorescein Diacetate)492517
CFDA (Carboxyfluorescein Diacetate)495520
Chinacrine450-495515
Cl-NERF514 (High pH)540 (High pH)
CyQuant GR480520
DCFH (Carboxydichlorofluorescein)505535
DiBAC4(3) (Bisdibutylbarbituric Acid)493516
DiO (Dipentyloxacarbocyanine Iodide)484502
DiOC16(3)484501
DiOC18(3)484501
DiOC2(3)482497
DiOC5(3)484500
DiOC6(3)484511
DiOC7(3)482504
DM-NERF510 (High pH) 497 (Low pH)536 (High pH) 527 (Low pH)
DTAF (Fluorescein Dichlorotriazine)494520
FAM (Carboxyfluorescein)492518
FDA (Fluorescein Diacetate)495520
FITC (Fluorescein Isothiocyanate)494518
FlCRhR (Cyclic AMP Fluorosensor)500517
Fluo-3480-506520
Fluo-4494516
Fluo-4FF494518
Fluo-5F494516
Fluo-5N493518
Fluorescein494518
Fluorescein Sulfonic Acid476519
FluoroEmerald495524
FluorX494520
FluoZin-1495517
FluoZin-2494521
FluoZin-3494516
GFP-RS (Red-Shifted)498516
LysoTracker Green DND-26504511
Magnesium Green506531
MitoFluor Green490516
MitoTracker Green FM490516
NeuroTrace 500/525500525
NeuroTrace 515/535515535
Newport Green DCF506535
Newport Green PDX490518
Nonyl Acridine Orange495519
Oligreen498518
Oregon Green 488496524
Oregon Green 500503522
Oregon Green 514511530
PFB-F (Pentafluorobenzoyl Fluorescein)492516
Phen Green FL492517
Phen Green SK507532
Pico Green502523
PKH67496520
Rhodamine 110496520
Rhodamine 123507529
Rhodamine Green502527
Sodium Green506532
SpectrumGreen 1497524
SpectrumGreen 2509538
Stachyose Fluorescein491516
SYBR Gold495537
SYBR Green I498522
SYBR Green II492513
SYTO 11508527
SYTO 12499522
SYTO 13488509
SYTO 16488518
SYTO 21494517
SYTO 23499520
SYTO 24490515
SYTOX Green504523
TAMRA (Carboxytetramethylrhodamine)504529
YFP/EYFP (Enhanced Yellow Fluorescent Protein)512529
YOYO-1, YO-PRO-1491509

 


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